16S rRNA Long-Read Sequencing of the Granulation Tissue from Nonsmokers and Smokers-Severe Chronic Periodontitis Patients

Table 1

Summary of the primer pair used to generate the 16S rRNA gene fragment fragments and the characteristics of each region in chronic periodontitis control and experimental samples.

(a)

Region		Forward Primers (with forward barcode)	Amplicon length (Without Barcode)
V1-V9	F	AGRGTTYGATYMTGGCTCAG	1,464
	F1	TGAGTGACGTGTAGCGAGRGTTYGATYMTGGCTCAG
	F2	GACAGCATCTGCGCTCAGRGTTYGATYMTGGCTCAG
	F3	TGCGAGCGACTCTATCAGRGTTYGATYMTGGCTCAG
	F4	TGCTCTCGTGTACTGTAGRGTTYGATYMTGGCTCAG

(b)

Region		Reverse Primers (with reverse barcode)	Amplicon length (Without Barcode)
V1-V9	R	RGYTACCTTGTTACGACTT	1,464
	R1	GCTCGACTGTGAGAGARGYTACCTTGTTACGACTT
	R2	TGCTCGCAGTATCACARGYTACCTTGTTACGACTT
	R3	GCAGACTCTCACACGCRGYTACCTTGTTACGACTT
	R4	AGACAGCATCTGCGCTCRGYTACCTTGTTACGACTT