T-614 Promotes Osteoblastic Cell Differentiation by Increasing Dlx5 Expression and Regulating the Activation of p38 and NF-κB

Figure 2

Calcium nodule formation in MSCs. MSCs were cultured in α-MEM supplemented with 10% FBS and 10 mM b-glycerophosphate in the absence or presence of 50 ng/ml rhBMP-2 and 10 μg/ml T-614 for 14 days. Then, the calcium content of the mineralized nodules in the cells was stained with Alizarin Red S at pH 4.3. (a) The data are representative of 3 separate experiments with similar results. (b) Statistical analysis of absorbance of Alizarin Red S staining. The data are expressed as the means +/− SD (). , compared with the vehicle control; , compared with the BMP2 group.

(a)

(b)