Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice

Figure 1

Improvement of glucose metabolism in GHSR knockout mice. (a) Body weight of GHSR knockout (GHSR-/-) mice and wild type (WT) mice fed with different diet. (b) The average daily food intake of GHSR-/- mice and wild type (WT) mice fed with normal chow diet or high fat diet for 12 weeks. (c) The average daily water intake of GHSR-/- mice and wild type mice fed with normal chow diet or high fat diet. (d) Results of glucose tolerance test using GHSR knockout mice and wild type mice fed with normal chow diet (NCD) or high fat diet (HFD) for 12 weeks. All results were expressed as means±SEM. (e) Western blotting results from GHSR knockout mice and wild type mice fed with normal chow diet or high fat diet for 12 weeks. Insulin at a dose of 2 IU/kg was injected intraperitoneally 15 min before harvest of the adipose tissue; phospho-Akt (Ser473) and Akt in adipose tissue were detected using specific antibodies. Total Akt level was used as internal control. Quantification of image analysis of p-Akt/Akt was expressed as means±SEM. denotes P<0.05 compared with wild type mice fed with NCD; ^# denotes P<0.05 compared with WT mice fed with HFD.

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