Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice

Figure 2

Improvement of adipose tissue inflammation in GHSR knockout mice. (a) Expression of inflammatory adipokines in epididymal adipose tissue of GHSR knockout mice, such as anti-inflammatory adipokine adiponectin and proinflammatory adipokines resistin, IL-6, and PAI-1. Relative mRNA levels were normalized to the levels for wild type mice fed with normal chow diet. Data are means±SEM, n=5. (b) Adipocytes volume and interstitial cell infiltration detected by H&E staining. (c) Macrophage infiltration detected by immunofluorescence histochemistry staining of Mac-3. denotes P<0.05 compared with WT mice fed with NCD; ^# denotes P<0.05 compared with WT mice fed with HFD.

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