Increased Autophagy Enhances the Resistance to Tumor Necrosis Factor-Alpha Treatment in Rheumatoid Arthritis Human Fibroblast-Like Synovial Cell

Figure 4

Cells were treated with TNF-α at concentrations 0, 10, 40, and 100 ng/ml for 48 h. LC3-II, Beclin1, and P62 were detected by western blot. Values are fold change, divided by the average value of relative optical density of these protein in the HFLS without any treatment. The data were analyzed and presented by histogram.