Validation of Housekeeping Genes as Reference for Reverse-Transcription-qPCR Analysis in Busulfan-Injured Microvascular Endothelial Cells

Figure 3

Graphical presentation of stability value by geNorm. (a) Showing the ranking of the 13 reference genes by geNorm software, with the most stable one toward the right and least stable one toward the left. (b) Determination of minimal number of reference genes by pairwise variation (Vn /n +1). The determination of optimal number of housekeeping genes by pairwise variation was shown. The effect of including more reference genes in a set of numbers of reference genes in all cases below the cut-off value of 0.15 is shown. The two most stable expressed reference genes may be accurate for qRT-PCR normalization. Including more reference genes for RT-qPCR normalization will not increase the stability of reference genes.

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