Research Article

Validation of Housekeeping Genes as Reference for Reverse-Transcription-qPCR Analysis in Busulfan-Injured Microvascular Endothelial Cells

Table 1

Summary of 14 housekeeping genes and target genes evaluated in this study. Official full name, accession numbers, official full name, primer sequences, and product sizes are shown.

SymbolOfficial full namePhysiological functionsAccession numbersPrimer sequence (forward/reverse)Products size (bp)

gapdhGlyceraldehyde-3-phosphate dehydrogenaseAn enzyme that catalyzed the sixth step of glycolysis, a process in which glucose is converted to pyruvate.NM_001001303F:catggccttccgtgttccta
R:gcggcacgtcagatcca
55 [2830]
actb Beta-actinProtein plays a key role in cell motility and cytoskeletal maintenance i.e. the structure and integrity.NM_007393F:atgtggatcagcaagcagga
R:aagggtgtaaaacgcagctca
99 [31]
ubcUbiquitin CProtein coded genes are involved in DNA repair and cell cycle regulation.NM_019639.4F:ccagtgttaccaccaagaag
R:acccaagaacaagcacaagg
94
eef1a1 eukaryotic translation elongation factor 1 alpha 1Translation elongation factorNM_010106F:tccgattacgacgatgttga
R:agtcgccttggacgttctt
125 [32]
b2mBeta -2 microglobulinThis gene encodes serum protein found on MHC class I on the surface of all nucleated cells.NM_009735F:ttcagtatgttcggcttccc
R:tggtgcttgtctcactgacc
103 [33, 34]
rplp0Ribosomal protein lateral stalk subunit P0It is a neutral phosphoprotein at the C-terminal end of ribosomal phosphoproteinsNM_007475F:ccgatctgcagacacacact
R:accctgaagtgctcgacatc
91 [35]
ywhaztyrosine 3-monooxygenase/tryptophan 5-monooxygenase
activation protein, zeta
A central hub protein for many signal transduction pathways and is a major regulator of apoptotic pathways.NM_011740F:ctttctggttgcgaagcatt
R:ttgagcagaagacggaaggt
148
hmbshydroxymethylbilane synthaseProviding instruction for making the enzyme hydroxymethylbilane synthase.NM_013551F:cagggtacaaggctttcagc
R:cggagtcatgtccggtaac
149 [36]
gusbβ-glucuronidaseProviding instruction for producing an enzyme called beta- glucuronidase.NM_010368F:actcctcactgaacatgcga
R:ataagacgcatcagaagccg
96 [37]
ppiaPeptidyl prolyl isomerase ACyclosporin binding protein /Inhibitor of serine threonine phosphataseNM_008907F:cagtgctcagagctcgaaagt
R:gtgttcttcgacatcacggc
109 [38]
tbpTATA box binding proteinProviding an instruction for making TAXA box binding proteins.NM_013684F:ggggtcataggagtcattgg
R:catctcagcaacccacacag
127 [39]
alas1δ-Aminolevulinate synthase 1catalyzing the first step of heme biosynthesisNM_020559F:gtctgtgccatctgggactc
R:ctgtccacatcagctgtcca
119
hprt1Hypoxanthine phosphoribosyltransferase 1providing instructions for producing an enzyme called hypoxanthine phosphoribosyl transferase 1NM_013556F:cataacctggttcatcatcgc
R:tcctcctcagaccgctttt
95 [40]
tfrctransferrin receptorThis gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis.NM_011638F:gcaccaacagctccaaagtc
R:ccagtgtgggaacaggtctt
133 [41]
ctnnb1catenin (cadherin associated protein), beta 1The key function of this protein is to mediate the canonical Wnt signaling pathway, regulate gene transcription and mediate cell-cell adhesionNM_007614.3F: gtgcgctgagcttcaggt
R: tcagctcgtgtcctgtgaag
147
robo4Roundabout guidance receptor 4Robo4 is a vascular-specific receptor.NM_028783.3F:cagcctggttagctcttctgatg
R:gcacgagcaaagtgagtatcagc
57 [42]
notch1Notch homolog 1It plays a role in a variety of developmental processes by controlling cell fate decisionsNM_008714.3F:ttcgtgctcctgttctttgtg
R:gggctctctccgcttcttc
129