Research Article
Validation of Housekeeping Genes as Reference for Reverse-Transcription-qPCR Analysis in Busulfan-Injured Microvascular Endothelial Cells
Table 2
Assay performance characteristics showing PCR coefficient (R2), slope, and primer pair efficiency (E).
| | Gene name | Coefficient (R2) | slope (A) | primer pair efficiency (E) |
| | actb | 1 | -3.482 | 0.937 | | gapdh | 0.99 | -3.402 | 0.968 | | eef1a1 | 0.997 | -3.282 | 1.017 | | rplp0 | 0.999 | -0.325 | 0.999 | | ppia | 0.999 | -0.452 | 0.948 | | ubc | 0.999 | -0.365 | 0.982 | | ywhaz | 1 | -3.37 | 0.906 | | hprt1 | 1 | -3.625 | 0.887 | | hmbs | 0.996 | -3.272 | 1.021 | | b2m | 0.998 | -3.405 | 1.021 | | tfrc | 1.0 | -3.215 | 0.966 | | alas1 | 0.989 | -3.502 | 1.047 | | gusb | 0.992 | -3.042 | 0.93 | | tbp | 1 | -3.37 | 1.131 |
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