Validation of Housekeeping Genes as Reference for Reverse-Transcription-qPCR Analysis in Busulfan-Injured Microvascular Endothelial Cells

Table 3

Mean Ct, STD, coefficient of variation, and Pearson coefficient of candidates’ RGs by BestKeeper.

Gene names	Geomean	Ar Mean	Min [CP]	Max [CP]	STD [±CP]	CV	Pearson coefficient [2]	p-value
ywhaz	24.33	24.51	22.17	32.58	2.37	9.66	0.996	0.001
actb	20.97	21.11	19.06	26.98	1.95	9.23	0.978	0.001
alas1	27.71	27.9	25.42	36.32	2.55	9.16	0.996	0.001
b2m	22.82	22.97	20.81	30.07	2.02	8.8	0.982	0.001
tfrc	27.15	27.32	25.2	35.58	2.29	8.4	0.99	0.001
ppia	23.15	23.27	20.73	28.96	1.92	8.26	0.962	0.001
hprt1	28.1	28.27	25.89	36.58	2.33	8.24	0.99	0.001
gapdh	21.13	21.23	19.62	26.18	1.7	8	0.958	0.001
rplpo	21.41	21.5	19.86	26.52	1.54	7.14	0.992	0.001
gusb	26.79	26.89	25.23	32.66	1.89	7.01	0.984	0.001
hmbs	28.83	28.94	26.98	35.4	2.03	7	0.994	0.001
ubc	23.13	23.24	21.04	29.42	1.59	6.85	0.835	0.002
tbp	28.15	28.2	26.93	32.47	1.22	4.32	0.96	0.001