Validation of Housekeeping Genes as Reference for Reverse-Transcription-qPCR Analysis in Busulfan-Injured Microvascular Endothelial Cells

Table 4

Expression stability detected by the comparative CT method (ΔΔCT method).

Gene names	Mean Ct value	SD	Variance	Ct±SD	Mean fold difference -CT	True fold difference log base-Ct
actb	21.105	2.672	7.139	-2.05±2.67	1.54±0.038	0.622
gapdh	21.348	2.085	4.97	-1.70±2.08	1.31±0.073	0.387
rplo	21.499	2.534	4.773	-1.65±2.53	1.45±0.055	0.540
ppia	23.373	2.534	6.423	-1.82±2.53	1.64±0.049	0.711
ubc	23.24	2.584	6.68	-2.21±2.58	1.31±0.036	0.379
ywhaz	24.512	3.462	11.986	-2.35±3.46	2.17±0.018	1.115
hprt1	28.266	3.519	12.383	-2.38±3.52	2.20±0.017	1.138
hmbs	28.939	2.842	8.077	-1.96±2.84	1.84±0.036	0.878
b2m	22.97	3.02	9.123	-2.16±3.02	1.82±0.028	0.86
tfrc	27.322	3.484	12.136	-2.13±3.48	1.33±0.020	0.408
Alas1	27.896	3.656	13.365	-2.48±3.66	2.26±0.014	1.175
gusb	26.894	2.587	6.693	-1.66±2.59	1.90±0.053	0.923
tbp	28.198	1.831	3.352	-1.27±1.83	1.47±0.012	0.558