Hydrocortisone’s effects on glucose uptake and glucose transporters in placental explants. (a) Glucose uptake as measured by 3H]-2-DOG incorporation. (b) GLUT1 mRNA expression normalized by actin mRNA expression. (c) GLUT3 mRNA expression normalized by actin mRNA. (d) GLUT1 protein levels as measured by western blot, normalized by actin levels, together with a representative photograph. Values are represented as means ± SEM for 5 independent experiments. p<0.05; p<0.025; p<0.01 relative to control (A.U.: arbitrary units).
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