miR-144-5p Enhances the Radiosensitivity of Non-Small-Cell Lung Cancer Cells via Targeting ATF2

Figure 2

miR-144-5p regulated A549 and H460 cell viability and apoptosis after irradiation. (a) The expression of miR-144-5p in control A549 and H460 cells (nontransfected cells), as well as cells transfected with agomir-144 or agomir-NC, was determined using qRT-PCR. (b) Control A549 and H460 cells as well as cells transfected with agomir-144 or agomir-NC were exposed to varying doses of radiation (0, 2, 4, 6, and 8 Gy). MTT assay was used to determine the cell viability 48 h after IR. Cell viability is expressed as the percentage relative to the control at 0 Gy. (c) A549 and H460 cells with or without agomir-144 or agomir-NC transfection were subjected to 8 Gy radiation. Cell apoptosis was assessed by staining with annexin V and propidium iodide 48 h after IR. The percentage of apoptotic cells was determined using flow cytometric analysis. Data are representative images or expressed as the mean ± standard deviation of each group of cells from three separate experiments. versus agomir-NC. versus agomir-NC.

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