Differential Expression of IL-36 Family Members and IL-38 by Immune and Nonimmune Cells in Patients with Active Inflammatory Bowel Disease

Figure 3

IL-36 production in immune and nonimmune intestinal cells from IBD patients. (a) CD14⁺/IL-36β expressing cells and (b) CD123⁺/IL-36β pDCs. Representative immunoperoxidase in inflamed tissue from active ulcerative colitis patients (n=10; left panel), active Crohn’s disease patients (n=10; middle panel), and noninflamed colonic tissue (n=10; right panel). Photomicrographs represent mucosa, submucosa, muscular, and serosa. Dotted arrows depict the cytokine expression, solid arrows show expression of cell-surface marker (leukocytes), double arrows indicate CD14⁺ or CD123⁺ (in brown)/IL-36β (in pink) double positive cells (burgundy), and circles highlight the immunoreactive perivascular inflammatory infiltrates. Original magnification was X320.

(a)

(b)