Differential Expression of IL-36 Family Members and IL-38 by Immune and Nonimmune Cells in Patients with Active Inflammatory Bowel Disease

Figure 4

IL-36 production in intestinal tissue from IBD patients. (a) CD8⁺/IL-36γ expressing cells. (b) CD123⁺/IL-36γ pDC. Representative immunoperoxidase in tissue from active ulcerative colitis patients (n=10; left panel), active Crohn’s disease patients (n=10; middle panel), and noninflamed colonic tissue (n=10; right panel). Photomicrographs represent mucosa, submucosa, muscular, and serosa. Dotted arrows depict the cytokine expression, solid arrows show expression of cell-surface marker (leukocytes), double arrows indicate CD8α⁺ or CD123⁺ (in pink)/IL-36γ (in brown) double positive cells (burgundy), and circles highlight the immunoreactive perivascular inflammatory infiltrates. Original magnification was X320.

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