Suppression of Th1 and Th17 Responses and Induction of Treg Responses by IL-18-Expressing Plasmid Gene Combined with IL-4 on Collagen-Induced Arthritis

Figure 3

Effect of various vectors on cytokine expression in the synovia of CIA mice. (a) Representative results showing changes in the expression of IL-17A, IL-18, IFN-γ, TNF-α, IL-10, FoxP3, T-bet, and GATA-3, determined by reverse transcription PCR analysis at 60 days after immunization. The lower part displays the density histogram data from three separated RT-PCR analyses (mean ± SE), which represents the relative expression of T-bet, GATA-3, TNF-α, IL-17A, IL-18, IFN-γ, IL-10, and FoxP3. (b) Changes in the expression of T-bet, GATA-3, TNF-α, IL-17A, IL-18, IFN-γ, IL-10, and FoxP3 were determined by real-time PCR. versus control by Student’s -test; versus control by Student’s -test.

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