Blockade of RBP-J-Mediated Notch Signaling Pathway Exacerbates Cardiac Remodeling after Infarction by Increasing Apoptosis in Mice

Figure 3

RBP-J knockout increased cardiomyocyte apoptosis following MI. (a) TUNEL staining showed that the number of apoptotic cells was increased in the border zone of ischemic heart tissue of Myh6-RBP- mice compared with RBP- mice with MI (original magnification: × 200, brown indicates TUNEL-positive nuclei and blue indicates nonapoptotic cells). The apoptosis rates of tissues in each group (Myh6-RBP--MI mice, RBP--MI mice and Myh6-RBP--sham mice, and RBP--sham mice) were detected (, ). Each value represents the mean ± SD. (b) Expression of bcl-2 and bax mRNA of different groups ((Myh6-RBP--MI mice, RBP--MI mice and Myh6-RBP--sham mice, and RBP--sham mice). (c) The protein expression level of bcl-2, bax, cleaved-caspase 3 by western blot analysis. The results are represented as mean ± SD (, , ).

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