Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in Polyphyllin I-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells

Table 1

Primer sequences and Tm for RT-qPCR.

Gene	Primer sequence (5′→3′)		reference
GRP78	F- TAGCGTATGGTGCTGCTGTC; R- TGACACCTCCCACAGTTTCA	=60°C	[21]
IRE-1	F- TGGATCCAAAACTACGCCTCC; R- GGTCAGATAGCGCAGGGTCTC	=58°C	[22]
ATF-6	F- TATCAGTTTACAACCTGCACCCACTA; R- GCAAGGACTGGCTGAGCAGA	=58°C	[22]
PERK	F- TTGTCGCCAATGGGATAG; R- CAGTCAGCAACCGAAACC	=58°C	[23]
CHOP	F- CAAAATCAGAGCTGGAACCTGAG; R- AGACCTTTCCTTTTGTCTACTCCAA	=60°C	[24]
TNFR1	F- TCCTTCACCGCTTCAGAAAA; R- GGGATAAAAGGCAAAGACCAA	=54°C	[25]
TNF-α	F- CCCAGGGACCTCTCTCTAATC; R- GCTACAGGCTTGTCACTCGG	=58°C	[26]
FAS	F- TCTGGTTCTTACGTCTGTTGC; R- CTGTGCAGTCCCTAGCTTTCC	=58°C	[25]
FASL	F- GCAGCCCTTCAATTACCCAT; R- CAGAGGTTGGACAGGGAAGAA	=60°C	[27]
β-actin	F- TGGCACCCAGCACAATGAA; R- CTAAGTCATAGTCCGCCTAGAAGCA	=60°C	[28]