JC Virus-DNA Detection Is Associated with CD8 Effector Accumulation in Peripheral Blood of Patients with Multiple Sclerosis under Natalizumab Treatment, Independently from JC Virus Serostatus

Figure 3

Evaluation of T-lymphocyte subset percentages in blood samples. Percentages of CD4+ (a) and CD8+ (b) T-lymphocyte subpopulations in peripheral blood of RRMS patients under natalizumab treatment are represented, after stratification according to natalizumab infusion number. Values are represented as percentage of the parental population (overall CD4 and CD8, resp.). Statistical analysis was performed using a one-way ANOVA test for nonparametrical data (Kruskal–Wallis, for CD4 N, ; CD4 CM, ; CD4 EM, ; CD4 E, ; CD8 N, ; CD8 I, > 0,05; CD8 CM, ; CD8 EM, ; CD8 E, ). Dunnett’s posttest was performed comparing each group with 0, and asterisks represent the level of statistical significance. Lines and whiskers represent median values and interquartile ranges, respectively. Dashed line represents median percentages observed in healthy donors. N: naïve, CM: central memory, EM: effector memory, E: effectors, and I: intermediate. 0: no infusions, 12: from 1 to 12 infusions, 24: from 13 to 24 infusions, 36: from 25 to 36 infusions, and : over 36 infusions of natalizumab. ; ; ; .

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