BioMed Research International / 2018 / Article / Tab 2 / Research Article
Antibiotics Resistance Genes Screening and Comparative Genomics Analysis of Commensal Escherichia coli Isolated from Poultry Farms between China and Sudan Table 2 Primer sequences and annealing temperatures used in the PCR reactions carried out the virulence genes of E. coli isolates in this study.
Antibiotics Resistances genes Sequence Size (bp) Annealing temp ( ) Reference genes Ampicillin ampC (F) AATGGGTTTTCTACGGTCTG (R) GGGCAGCAAATGTGGAGCAA 191 Woodford et al. [10 ] Tetracycline tet(A) (F) GGTTCACTCGAACGACGTCA (R) CTGTCCGACAAGTTGCATGA 577 57 Woodford et al. [10 ] Kanamycin pKD13 (F) AGGCTTTTGCTTGAATGTTCCGTCAAGGGATCAC GGGTAGGAGCCACCTTGTGTAGGCTGGAGCTGCTTC (R) GGTCGACGGATCCCCGGAATGTAAGCATCTGTC AGAAAGGCCAGTCTCAAGCGAGGCTGGCCTTTTCTGT Woodford et al. [10 ] Wright [9 ] Erythromycin erm(B) (F) GAAAAAGTACTCAACCAAATA (R) AATTTAAGTACCGTTAC 642 45 Woodford et al. [10 ] erm(A) (F) TCTAAAAAGCATGTAAAAGAAA (R) CGATACTTTTTGTAGTCCTTC 533 52 Woodford et al. [10 ] erm(C) (F)TCAAAACATAATATAGATAAA (R)GCTAATATTGTTTAAATCGTCAAT 642 45 Wright [9 ] Doxycycline tet(B) (F) CCTCAGCTTCTCAACGCGTG (R) GCACCTTGCTGATGACTCTT 634 56 Woodford et al. [10 ] Spectinomycin aadA14 (F) GTGAGGAGGAGCTTCGCGAG (R) TGCCGCAGGACTCGGAGGTC 642 60 Woodford et al. [10 ] cefotaxime mph(A) (F) GTGAGGAGGAGCTTCGCGAG (R) TGCCGCAGGACTCGGAGGTC 403 60 Wright [9 ] mph(B) (F)GATATTAAACAAGTAATCAGAATAG (R) GCTCTTACTGCATCCATACG 494 58 Wright [9 ] Ciprofloxacin acrA (R) TGCAGAGGTTCAGTTTTGACTGTT (F) CTCTCAGGCAGCTTAGCCCTAA 107 Velicer et al. [11 ] Gentamicin aac(3)-I (F) ACCTACTCCCAACATCAGCC (R) ATATAGATCTCACTACGCGC 169 60 Velicer et al. [11 ] aac(3)-III (F) CACAAGAACGTGGTCCGCTA (R) AACAGGTAAGCATCCGCATC 185 60 Velicer et al. [11 ] Streptomycin aadA1 (F) TATCCAGCTAAGCGCGAACT (R) ATTTGCCGACTACCTTGGTC 286 55 Woodford et al. [10 ]