Immobilization of a Laccase/2,2’-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic Acid System to Layered Double Hydroxide/Alginate Biohybrid Beads for Biodegradation of Malachite Green Dye

Figure 6

(a) Effects of ZnCr-ABTS LDH content in alginate beads on dye decolorization. (b) Effects of pH on dye decolorization by the free LMS (Lac/ABTS) and Im-LMS (Lac/ZnCr-ABTS LDH/alginate beads). (c) Dye decolorization by the free LMS, the Im-LMS, and laccase alone as a function of time. The decolorization conditions were as follows: 20 mg/L dye, 30 mg Im-LMS, or 1200 U/L laccase+2.1 mM ABTS or 1200 U/L laccase in 4.0 mL acetate buffer (pH 6.0). (d) Reusability of the Im-LMS (30 mg) over eight successive cycles using 20 mg/L dye in acetate buffer at pH 6.0, with shaking at 100 rpm for 2 h at 25°C.

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