Oncogenic N-Ras Stimulates SRF-Mediated Transactivation via H3 Acetylation at Lysine 9

Figure 2

Oncogenic N-RasN12 stimulates SRE transactivation by mediating RGL2. (a) CV-1 cells were grown in a 12-well plate and transfected with the indicated reporter plasmids along with expression vectors for oncogenic H/K/N-Ras. Expression of oncogenic Ras isoforms was monitored by Western Blotting with anti-HA antibody. (b) The luciferase activity was determined 24 hr after transfection, and the relative activities of three independent experiments are shown as mean ± SD. (c) CV-1 cells were transfected with SRE-Luc reporter and expression vectors for oncogenic H/K/N-Ras together with mock, RalGDS-RBD, or RGL2-RBD plasmids as indicated. The luciferase activity was determined as shown in (b) and results show the mean ± SD of three experiments.

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