Oncogenic N-Ras Stimulates SRF-Mediated Transactivation via H3 Acetylation at Lysine 9

Figure 4

Oncogenic N-Ras-mediated H3 acetylation at lysine 9 is critical for target gene transcription. (a) 293T cells were transfected with the indicated expression plasmids for 48 hr. Total RNA was prepared from cells and qRT-PCR was performed using primers specific for Cyr6, Egr-1, JunB, Scyl1, E2F5, and Npm1 genes. The results shown are mean values from three independent experiments, and values derived from -Actin in empty vector transfected cells are arbitrarily set to 1. (b) ChIP assays of JunB and Egr-1 genes were performed using anti-H3K9ac and anti-H3 antibodies in 293T cells expressing Ras isoforms as in (a). ChIP-enriched DNA was determined for promoter and coding region by qPCR using the indicated primers. The data are the mean of three independent experiments ± SD.

(a)

(b)