Identification of Candidate Biomarkers in Malignant Ascites from Patients with Hepatocellular Carcinoma by iTRAQ-Based Quantitative Proteomic Analysis

Figure 1

Differentially distributed proteins identified by iTRAQ quantitative analysis. (a) Volcano plots showing differentially distributed proteins comparing HCC and HD. Each point represents the difference of fold-change (X axis, Log₁₀[fold change]) plotted against the level of statistical significance (Y axis, Log₁₀[p value]). Proteins represented by red dots indicate upregulation and green dots indicate downregulated proteins (p<0.05, ratio >1.3 or < 0.767). (b) Hierarchical clustering of the differentially distributed proteins identified from each samples (ratio >1.3 or < 0.767). The color scale indicates the expression level of each protein across the two groups. (c) ELISA assay for carbonic anhydrase I (CA1) in the ascitic fluid samples.

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