γδT Cells Exacerbate Podocyte Injury via the CD28/B7-1-Phosphor-SRC Kinase Pathway

Figure 3

(a) WT mice were treated with LPS, and CD28⁺γδT cells in kidney were detected by flow cytometry at 0, 1, 3, 5, and 7 days after LPS treatment (). CD28⁺γδT cells decreased significantly from the first day (). (b) Podocytes were treated with LPS (50 ug/ml and 100 ug/ml); and . Quantitative-RT-PCR detection result revealed that compared to control group, podocytes treated with LPS had higher B7-1 RNA expression and increased positively with LPS concentration. (c) Western blotting detection showed higher B7-1 expression in podocytes treated with LPS (100 ug/ml) than control group; . (d) B7-1 gene expression in kidney tissue of nephrotic WT mice group was threefold higher than that of control group (, ). (e) Double labelling with synaptopodin (synpo) showed B7-1 expression was restricted to podocytes. (f) Immunofluorescence image showed B7-1 (red) and γδT cells (green) were detected in glomeruli of nephrotic WT mice group. And their position was near and even overlapped. For experiments in vitro, all measurements were performed in duplicate.

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