Cytokine Effect of TLR3, TLR4, and TLR7 Agonists Alone or Associated with Leishmania infantum Antigen on Blood from Dogs

Table 2

Results of TNF-α and IL-6 concentrations after stimulation at each condition in all dogs studied (n=24).

Cytokines (pg/mL)	Treatment conditions (mean ± SD)
		+LSA	+ConA	+TLR3a	+TLR4a	+TLR7a	LSA+TLR3a	LSA+TLR4a	LSA+TLR7a
TNF-	9.6±17.1	27.1±32.7	107.7±107.7	17.2±28.0	116.3±10.9	92.0±56.1	18.8±17.2	116.1±73.8	157.1±78.1
IL-6	15.1±19.5	12.6±19.0	24.3±22.7	17.0±26.8	76.7±39.9	55.1±32.2	16.9±20.4	80.6±39.3	75.4±39.7

Mean ±standard deviation (mean ± SD), medium alone (Φ); Leishmania soluble antigen (LSA); Concanavalin A (ConA); TLR3 [Poly(I:C)] agonist (Φ+TLR3a) and TLR3a and LSA (LSA+ TLR3a); TLR4 (MPLA) agonist (Φ+TLR4a) and TLR4a and LSA (LSA+ TLR4a); TLR7 (IMQ) agonist (Φ+TLR7a) and TLR7a and LSA (LSA+ TLR7a)