Small Molecule Inhibitor of Type Three Secretion System Belonging to a Class 2,4-disubstituted-4H-[1,3,4]-thiadiazine-5-ones Improves Survival and Decreases Bacterial Loads in an Airway Pseudomonas aeruginosa Infection in Mice

Figure 2

Treatment with FT reduces P. aeruginosa loads after infection with antibiotic-resistant P. aeruginosa clinical isolates of exoU⁺ (1840) and exoS⁺(KB6) genotypes. A/Sn mice were infected with 6.5х10⁶ CFU/mouse of P. aeruginosa clinical isolate 1840 ((a), group 1); with 3.25х10⁶ CFU/mouse of P. aeruginosa clinical isolate 1840 ((b), group 2); with 2.2x10⁷ CFU/mouse of P. aeruginosa clinical isolate KB6 ((c), group 1); with 1.1х10⁷ CFU/mouse of P. aeruginosa⁺ clinical isolate KB6 ((d), group 2). Infected animals were treated per os with 50 mg/kg of FT immediately after infection for 4 days twice a day. Bacterial loads in lungs, spleens, and blood of survived animals were analyzed at day 5 postinfection. Black bars, mice not treated with FT; dotted bars, mice treated with FT, P < 0.05.

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