Small Molecule Inhibitor of Type Three Secretion System Belonging to a Class 2,4-disubstituted-4H-[1,3,4]-thiadiazine-5-ones Improves Survival and Decreases Bacterial Loads in an Airway Pseudomonas aeruginosa Infection in Mice

Figure 4

The prophylaxis-therapy regimen with FT reduces P. aeruginosa loads after infection with antibiotic-resistant P. aeruginosa clinical isolates of exoU⁺ (1840) and exoS⁺(KB6) genotypes. A/Sn mice were infected with 7x10⁶ CFU/mouse of P. aeruginosa clinical isolate 1840 ((a), group 1); with 3.5x10⁶ CFU/mouse of P. aeruginosa clinical isolate 1840 ((b), group 2); with 1.75x10⁷ CFU/mouse of P. aeruginosa clinical isolate KB6 ((c), group 1); with 8x10⁶ CFU/mouse of P. aeruginosa clinical isolate KB6 ((d), group 2). Mice were treated with 100 mg/kg of FT per os once a day for 2 days before infection and with 50 mg/kg of FT twice a day for 4 days starting immediately after infection. Bacterial loads in lungs, spleens, and blood of survived animals were analyzed at day 5 postinfection. Black bars, mice not treated with FT; dotted bars, mice treated with FT, P < 0.05.

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