Small Molecule Inhibitor of Type Three Secretion System Belonging to a Class 2,4-disubstituted-4H-[1,3,4]-thiadiazine-5-ones Improves Survival and Decreases Bacterial Loads in an Airway Pseudomonas aeruginosa Infection in Mice

Figure 9

FT increased bacteria internalization in a dose-dependent manner. To assess the capability of FT to affect bacteria internalization, P. aeruginosa exoS⁺ clinical isolate 1653, sensitive to gentamicin, was preincubated with FT for 30 min and was added to HeLa cells at MOI of 10. After incubation for 2 hours extracellular bacteria were eliminated by gentamicin. The numbers of intracellular bacteria were determined 2 hours later, P < 0.05.