Deferoxamine but Not Dimethyloxalylglycine, L-Mimosine, or Cobalt Dichloride Can Interfere with the MTT Assay

Figure 3

The impact of hypoxia mimetic agents, when washed or unwashed, measured in the MTT assay without the presence of MC3T3-E1 in phosphate buffered saline. The MTT assay was performed in experiments without MC3T3-E1 cells with dimethyloxalylglycine (DMOG), deferoxamine (DFO), L-mimosine (L-Mimo), or CoCl₂ in the presence of phosphate buffered saline (PBS). The plates were either left unwashed before adding the MTT (a) or were washed with PBS before addition of MTT (b). PBS only served as control.