Clinically Correlated MicroRNAs in the Diagnosis of Non-Small Cell Lung Cancer: A Systematic Review and Meta-Analysis

Table 1

The main features of 71 included studies in this meta-analysis.

Study ID	Ethnicity	Specimen	Case	Age	Control	Age	Type of control	Stage	MiRNA profiling	SEN ()	SPE ()	Reference miRNA	microRNA assay	Smoking status
			N		N
Zhang H 2017	Asian	plasma	129	59.6	83	60.0	HC	I-II	miR-145, miR-20a, miR-21, miR-223	81.8	90.1	miR-16	qRT-PCR	3
Halvorsen A 2016	Caucasian	serum	100	62.6	58	57.6	HC	I-IV	miR-429, miR-205, miR-200b, miR-203, miR-12, miR-34b	88.0	71.0	miR-220, miR-19b, U6	qRT-PCR	3
TaiMei C2016	Asian	blood	110	65.0	52	65.7	HC	I-III	20 miRNAs	89.1	100	miR-159a, U6	qRT-PCR	4
Su KL 2016	Asian	plasma	100	NA	100	NA	HC	I-III	miR-195	78.0	86.0	miR-39	qRT-PCR	3
Zhu WY 2016	Asian	plasma	112	58.5	40	57.9	HC	I-III	miR-182, miR-183, miR-210, miR-126	81.3	100	U6	qRT-PCR	2
Jiang LP 2016	Asian	tissue	154	54.9	63	57.8	BPD	I-IV	miR-26b	79.9	79.4	U6	qRT-PCR	3
Wang Y 2016	Asian	plasma	82	NA	91	NA	HC	I-II	miR-532, miR-628, miR-425	91.5	97.8	miR-39	qRT-PCR	4
Wang Y 2016	Asian	plasma	36	NA	43	NA	HC	I-II	miR-532, miR-628, miR-425	97.2	95.3	miR-39	qRT-PCR	4
Fan LH 2016	Asian	serum	94	60.5	58	58.1	HC	I-III	miR-15b, miR-16, miR-20a	86.2	91.4	NA	qRT-PCR	4
Fan LH 2016	Asian	serum	70	59.7	54	58.0	HC	I-III	miR-15b, miR-16, miR-20a	94.3	94.2	NA	FQDs	4
Sun L 2016	Asian	plasma	87	60.7	96	53.8	HC,BPD	I-IV	miR-30a	61.0	84.3	U6	qRT-PCR	4
Su Y 2016	Asian	sputum	144	66.3	171	65.2	BPD	I	miR-21, miR-31, miR-210	81.5	85.9	U6	qRT-PCR	1
Gao X 2016	Asian	plasma	30	61.1	30	60.2	HC	I	miR-324, miR-1285	93.3	90.0	miR-39	qRT-PCR	4
Wang X 2016	Asian	plasma	59	55.9	59	57.6	BPD	I-III	miR-486	83.1	78.0	miR-16	qRT-PCR	4
Wei J 2016	Asian	plasma	63	61.0	30	57.0	HC	I-IV	miR-21	76.2	70.0	miR-16	qRT-PCR	3
Razzak 2016	Caucasian	sputum	22	68	10	58	HC,BPD	III-IV	miR-21, miR-210, miR-372	64	100	U6	qRT-PCR	4
Razzak 2016	Caucasian	sputum	21	70	10	58	HC,BPD	I-II	miR-21, miR-210, miR-372	67	90	U6	qRT-PCR	4
Leidinger P 2016	Caucasian	blood	74	NA	20	NA	HC	I-III	miR-720, miR-29c, miR-199a, miR-378a,let-7f	91.0	98.0	U24,U48	qRT-PCR	4
Wang WZ 2016	Asian	tissue	15	57	16	58	HC	I-IV	miR-182, miR-10a, miR-301b, miR-1244, miR-301a, miR-135b, miR-224, miR-21	93.3	93.8	miR-16	qRT-PCR	4
Wang WZ 2016	Asian	serum	54	NA	15	NA	HC	I-IV	miR-1244	81.5	80	miR-39	qRT-PCR	4
Kim JL O 2015	Caucasian	BAL	21	70	10	59	HC,BPD	I-II	miR-21, miR-143, miR-155, miR-210, miR-373	85.7	100	U6	qRT-PCR	4
Wang C 2015	Asian	serum	19	61.8	19	62.1	HC	I-IV	miR-483, miR-193a, miR-25, miR-214, miR-7	100	84	let-7d/g/i	qRT-PCR	4
Li WS 2015	Asian	plasma	11	59	11	55	HC	I-III	mir-486	90.9	81.8	miR-39, U44	qRT-PCR	4
Wang C 2015	Asian	serum	63	61.9	63	59.7	HC	I-IV	miR-483, miR-193a, miR-25, miR-214, miR-7	89.0	68.0	let-7d/g/i	qRT-PCR	4
Wang C 2015	Caucasian	serum	108	67.2	56	63.7	BPD	I-IV	miR-483, miR-193a, miR-25, miR-214, miR-7	95.0	95.0	let-7d/g/i	qRT-PCR	4
Wang C 2015	Caucasian	serum	108	67.2	48	58.5	HC	I-IV	miR-483, miR-193a, miR-25, miR-214, miR-7	95.0	84.0	let-7d/g/i	qRT-PCR	4
Nadal E 2015	Caucasian	serum	70	67.5	22	67.0	HC,BPD	I-III	miR-141, miR-200b, miR-193b	96.0	95.0	U6	qRT-PCR	2
Nadal E 2015	Caucasian	serum	84	65.5	23	60.0	HC,BPD	I-III	miR-141, miR-200b, miR-193b	97.0	96.0	U6	qRT-PCR	2
Guo WG 2015	Asian	plasma	126	NA	50	NA	HC	I-IV	mir-204	76.0	82.0	U6	qRT-PCR	4
Ma J 2015	Caucasian, African	PBMC	84	64.1	69	62.4	BPD	I-IV	miR-19b, miR-29b	72.6	82.6	miR-423-3p	qRT-PCR	2
Li L 2015	Asian	serum	36	56.0	30	58.0	HC,BPD	I-IV	miR-148a, miR-148b, miR-152	72.2	90.0	U6	qRT-PCR	4
Zhang XL 2015	Asian	tissue	125	61.0	125	61.0	HC	I-IV	miR-141	64.8	64.8	miR-191, miR-103	qRT-PCR	3
Zhao W 2015	Asian	serum	80	57.6	60	55.4	HC	NA	miR-21	73.8	71.7	U6	qRT-PCR	4
Wang RJ 2015	Asian	serum	70	64.4	70	63.7	HC	NA	miR-145	92.8	61.4	miR-39	qRT-PCR	3
Yang JS 2015	Asian	serum	152	NA	300	NA	HC	I-IV	miR-152, miR-148a, miR-148b, miR-21	96.0	91.0	U6	qRT-PCR	3
Xing LX 2015	Caucasian	sputum	67	66.4	69	64.9	BPD	I-II	miR-21, miR-31, miR-210	82.1	88.4	U6, miR-16	qRT-PCR	4
Liu CM 2015	Asian	Pleural effusion	61	53.8	70	54.4	BPD	NA	miR-192	61.3	79.5	U6	qRT-PCR	2
Dou HL 2015	Asian	plasma	120	63.2	360	NA	HC	I-IV	miR-152	86.0	81.3	U6	digital PCR	4
Yang YL 2015	Asian	PBMC	74	62.5	52	61.8	HC	I-IV	miR-10b	86.5	76.9	miR-16	qRT-PCR	3
Li N 2014	Caucasian, African	sputum	35	68.9	40	65.7	HC	I	miR-31, miR-210	65.7	85.0	NA	qRT-PCR	4
Zhu W 2014	Asian	serum	70	59.0	48	NA	HC	I-IV	miR-429	54.3	81.2	U6,U48	qRT-PCR	4
LI M 2014	Asian	serum	514	NA	54	NA	HC	I-IV	miR-499	73.7	92.7	miR-39	qRT-PCR	3
Ulivi P 2013	Caucasian	blood	86	68.0	24	65.0	HC	I-II	miR-328	70.0	83.0	U38B,U58A	qRT-PCR	4
Bediaga 2013	Caucasian	tissue	45	66.4	45	66.4	HC	I-IV	8 miRNAs	100	97.8	4miRNAs	qRT-PCR	3
Bediaga 2013	Caucasian	tissue	47	67.8	47	67.8	HC	I-IV	8 miRNAs	97.5	96.3	4miRNAs	qRT-PCR	3
Bediaga 2013	Caucasian	tissue	22	68.4	22	68.4	HC	I-IV	8 miRNAs	100	95.0	4miRNAs	qRT-PCR	3
Anjuman 2013	Caucasian, African	sputum	39	65.6	42	62.3	BPD	I	miR-210, miR-31	61.5	90.5	U6	qRT-PCR	4
Tang DF 2013	Asian	plasma	62	64.8	60	66.0	HC	I-III	miR-21, miR-145, miR-155	69.4	78.3	U6	qRT-PCR	1
Tang DF 2013	Asian	plasma	34	65.2	32	66.4	HC	I-III	miR-21, miR-145, miR-155	76.5	81.3	U6	qRT-PCR	1
Mozzoni 2013	Caucasian	plasma	54	69.1	46	64.1	BPD	I-III	miR-21, miR-486	87.0	86.5	miR-16	qRT-PCR	4
ZENG XL 2013	Asian	PBMC	64	58.9	26	54.4	HC	I-IV	miR-143	75.0	92.3	U6	qRT-PCR	4
Yang XQ 2013	Asian	sputum	24	60.5	24	57.8	BPD	I-IV	let-7a	87.5	83.3	U6	digital PCR	4
Ma J 2013	Caucasian, African	plasma	36	66.7	38	64.6	HC	I	miR-21, miR-335	71.8	80.6	NA	qRT-PCR	4
Cazzoli R 2013	Caucasian, African	plasma	50	66.1	30	64.8	BPD	I	miR-151a, miR-30a, miR-200b, miR-629, miR-100, miR-154	96.0	60.0	let7a	qRT-PCR	2
Abd-E 2013	African	Serum	65	54.1	37	50.1	HC	I-II	miR-182	100	86.5	SNORD68	qRT-PCR	4
Sanfiorenzo C 2013	Caucasian	plasma	52	65.1	10	68.9	BPD	I-III	miR-152, miR-145, miR-199a, miR-24, miR-20a, miR-25	90.9	83.3	miR-192, miR-16	qRT-PCR	4
Roa Wilson H 2012	Caucasian	sputum	24	68.8	6	44.7	HC	I-II	miR-21, miR-143, miR-155, miR-210, miR-372	83.3	100	U6	qRT-PCR	4
Li GJ 2012	Asian	plasma	16	NA	14	NA	BPD	I	miR-494, miR-22, miR-200b	85.3	94.5	18S	qRT-PCR	4
Ma YX 2012	Asian	serum	193	NA	110	NA	HC	I-IV	miR-125b	78.2	66.4	NA	qRT-PCR	4
Hennessey P 2012	Caucasian, African	serum	55	68.2	75	65.7	HC	I-IV	miR-15b, miR-27b	100	84.0	miR-16	qRT-PCR	4
ZengXL 2012	Asian	PBMC	34	NA	26	54.4	HC	I-IV	miR-150	87.5	69.2	U6	qRT-PCR	4
Zhao M 2012	Asian	tissue	55	NA	55	NA	HC	I-IV	miR-29a	49.1	85.5	U6	qRT-PCR	3
Shen J 2011	Caucasian, African	plasma	34	68.0	29	66.0	HC	I-IV	miR-21, miR-126, miR-210, miR-486	91.7	96.6	miR-16	qRT-PCR	1
Jeong H 2011	Asian	blood	35	67.0	30	60.0	HC	I-IV	let-7a	90.3	90.3	U6	qRT-PCR	4
Wei J 2011	Asian	plasma	77	59.6	36	56.4	HC	I-IV	miR-21	61.0	83.3	miR-16	qRT-PCR	3
Liu S 2011	Asian	plasma	130	53.1	170	57.5	HC	I-III	miR-126	46.4	90	NA	qRT-PCR	3
Yu L 2010	Caucasian, African	sputum	36	68.2	36	66.7	HC	I	miR-486, miR-21, miR-200b, miR-375	80.6	91.7	U6	qRT-PCR	4
Yu L 2010	Caucasian, African	sputum	64	67.0	58	65.0	HC	I-IV	miR-486, miR-21, miR-200b, miR-375	70.3	80.0	U6	qRT-PCR	3
Xing LX 2010	Caucasian, African	sputum	48	67.5	48	65.9	HC	I	miR-205, miR-210, miR-708	73.0	96.0	U6	qRT-PCR	4
Xing LX 2010	Caucasian, African	sputum	67	68.0	55	65.0	HC	I-IV	miR-205, miR-210, miR-708	72.0	95.0	U6	qRT-PCR	3
Keller Andreas 2009	Caucasian	blood	17	64.2	19	37.9	HC	I-III	24miRNAs	92.5	98.1	NA	qRT-PCR	4

miR-451, miR-1290, miR-636, miR-30c, miR-22-3p, miR-19b, miR-486-5p, miR-20b, miR-93, miR-34b, miR-185, miR-126-5p, miR-93-3p, miR-1274a, miR-142-5p, miR-628-5p, miR-486-3p, miR-425, miR-645, miR-24; miR-96, miR-450a, miR-183, miR-9, miR-577, Let-7i, miR-27b and miR-34a; miR-26a, miR-140-5p, miR-195, miR-30b;miR-126, miR-423, miR-15a, let-7d, let-7i, miR-22, miR-98, miR-19a, miR-20b, miR-324, miR-574, miR-195, miR-25, let-7e, let-7c, let-7f, let-7a, let-7g, miR-140, miR-339, miR-361, miR-1283, miR-18a, miR-26b; 1: only smokers; 2: smokers and nonsmokers (smoking status was imbalanced between groups); 3: smokers and nonsmokers (smoking status was balanced between groups); 4: unknown smoking status.
N: number; HC: healthy control; BPD: benign pulmonary disease; miR: microRNA; SEN: sensitivity; SPE: specificity; FQDs: fluorescence quantum dots; BAL: bronchoalveolar lavage.