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BioMed Research International
Volume 2018 (2018), Article ID 5961974, 6 pages
https://doi.org/10.1155/2018/5961974
Research Article

PTX3: A Potential Biomarker in Thyroid Associated Ophthalmopathy

1Department of Ophthalmology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
2Department of Ophthalmology, Shanghai Zhabei District Central Hospital, Shanghai 200003, China

Correspondence should be addressed to Jinwei Cheng; moc.361@evawnnij and Ruili Wei; moc.621@iewiliur

Received 3 October 2017; Revised 17 January 2018; Accepted 23 January 2018; Published 20 February 2018

Academic Editor: Yoshifumi Saisho

Copyright © 2018 Pei Mou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Thyroid associated ophthalmopathy (TAO) is an autoimmune disease, which involves inflammation and tissue remodeling. Pentraxin-3 (PTX3) is a component of innate immune system and recently implicated in autoimmunity. This observation may indicate that PTX3 participates in the inflammatory process of TAO. Methods. All studies were performed on TAO patients and healthy controls (45: 28 in total). RNA-seq was used to detect differential gene expression of orbital adipose-connective tissue. Quantitative PCR was performed to verify the results. PTX3 protein in orbital adipose-connective tissues was visualized by immunohistochemistry (IHC). PTX3 concentration in serum was determined by enzyme-linked immunosorbent assay (ELISA). Results. RNA-seq showed 1.86- higher PTX3 expression in the orbital adipose-connective tissues from TAO group than controls (FDR = 0.0059). qPCR confirmed the difference (5.59-fold increase, ). The presence of PTX3 protein was demonstrated. Orbital adipose tissue from healthy controls showed weak staining for PTX3 while tissue from TAO group was strongly positive. Serum PTX3 concentration was significantly elevated in patients when compared to the control group (1.9-fold increase; ). Conclusions. Patients with TAO showed increased presence of PTX3 in orbital tissue and serum, which may suggest a potential relationship of PTX3 and TAO.