Effects of Vitamin D Restricted Diet Administered during Perinatal and Postnatal Periods on the Penis of Wistar Rats

Figure 1

Photomicrographs of connective tissue, sinusoidal spaces, and smooth muscle in corpus cavernosum and corpus spongiosum of offspring penis at 4 months of age. (a) Corpus cavernosum; arrows indicate an increase of percentage of connective tissue and arrow head shows a reduction of sinusoidal spaces in VitD/VitD. Masson’s trichrome, ×400. (b) Corpus spongiosum; arrows demonstrate an increase of percentage of connective tissue in VitD/VitD. Masson’s trichrome, ×1000; arrows show an increased percentage of smooth muscle in corpus cavernosum (c) and in corpus spongiosum (d) in VitD/VitD. Immunostaining for anti-alpha smooth muscle actin, (c) ×400 and (d) ×1000, respectively.

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