Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2

Figure 3

MiR-29b downregulates the expression of Mcl-1 and CCND2. (a and b) The potential binding sites of miR-29b on the 3′ UTR of Mcl-1 and CCND2. (c) The expression of Mcl-1 and CCND2 in PASMCs treated with the miR-29b mimic, inhibitor, or control by Western blot. (d and e) The effect of the miR-29b mimic and inhibitor on the wildtype and mutant 3′ UTR of Mcl-1 as analyzed by a luciferase assay. (f and g) The effect of the miR-29b mimic and inhibitor on the wildtype and mutant 3′ UTR of CCND2 as analyzed by a luciferase assay. and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)