Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2

Figure 4

Mcl-1 and CCND2 silencing results in increased PASMC apoptosis and rescues the PASMC G1/S delay, respectively, in response to miR-29b expression. (a and c) The representative graph and statistical chart of the PASMC apoptosis rate for each indicated treatment for 48 h after si-Mcl-1 transfection as determined by flow cytometry following AnnexinV and PI staining. (b and d) Representative dot plots and statistical chart of the number of PASMCs treated with the miR-29b mimic, inhibitor, or negative control for 48 h after si-CCND2 transfection in the G0/G1, S, and G2/M phases.

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