Ubiquitin-Mimicking Peptides Transfer Differentiates by E1 and E2 Enzymes

Figure 2

(a) ATP-PPi exchange rates of wild-type ubiquitin and CP1, CP2, and CP7 proteins with Ube1. ^CP1P<0.05 versus wt ub, ^CP2P<0.05 versus wt ub, and ^CP7P<0.05 versus wt ub, n=3. (b) Western-blot of E1 transfer of wild-type ubiquitin and CP1, CP2, and CP7 proteins with Ube1.

(a)

(b)