Ubiquitin-Mimicking Peptides Transfer Differentiates by E1 and E2 Enzymes

Table 2

Kinetic parameters of ATP-PPi exchange catalyzed by Ube1 and peptides. ^aP<0.05 versus Ube1+P1, ^bP<0.05 versus Ube1+P1, and ^cP<0.05 versus Ube1+P1, n=3.

	(μM)	(min^−1)	(μM min^−1)
Ube1+ P1	ND	ND	5.24x 10^−5
Ube1+C1	651.4±49.3	2.29±0.59	3.52 x
Ube1+C2	611.1±23.1	3.73±0.87	6.10 x
Ube1+C7	542.6±122.1	5.54±1.37	1.01 x
Ube1+ P3	476.1±163.2	6.68±0.77	1.40 x10^−2
Ube1+ P4	250.9±55.6	13.98±2.08	5.57x10^−2