Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Volvariella volvacea Gene Expression under Different Conditions

Table 1

Candidate reference genes evaluated for expression stability in V. volvacea.

Gene name	Description	Accession	Primer sequence: forward/reverse(5′-3′)	Length (bp)	PCR efficiency	R^2
ACTB	beta-actin	KF528321	TATCGATAATGGCTCCGGCATGTGC/ATACCACGCTTGGATTGGGCCTCAT	165	116.629	0.998
CYP	cyclophilin	KF528322	AGAATGGCTTTGGATACAAGGGGTC/CCTGAAGTTCTCATCTGCGAATCTCTC	140	114.960	0.998
GAPDH	glyceraldehyde-3-phosphate dehydrogenase	KF528323	GATGCTTACGATCCCAAGTACACCG/CTACGACCACCACGCCAATCTTT	191	112.199	1
TUBα	alpha-tubulin	KF528325	GAGCCCAATGTTATCGATGAAGTGC/GTTCTTTGCCAATTGTGTAGTGCCC	130	110.336	0.999
TUBβ1	beta-tubulin 1	KF525326	GTTGATTTGGAGCCTGGAACTATGG/TCCTTCCGTATAGTGTCCTTTTGCC	132	128.813	0.997
UBQ	ubiquitin	KF528328	CAATCACCTTGGAAGTCGAGTCGTC/CTGGATGTTGTAGTCGGAAAGGGTG	152	107.601	1
MSF1	MSF1-domain-containing protein	KF528329	TCTGTCGACCCCACAACTGGCATAA/TCTGTGTAGCTGGGTCGACGAATGA	145	111.670	0.998
SPRYp	SPRY domain protein	KF528330	GCATTCTTCTTGATGTCGGTGGTCG/AACCCTGAAGTGTTGGATGCTCTGG	130	115.097	0.997
Lasp	L-asparaginase	KF528333	GTCACGTCAAGCCTCAAACCAAAAC/ATCGAATAGACTTCATACCACCTCCCC	157	109.315	0.997
MAPK	mitogen-activated protein kinase	FJ906769	TCCGAACACAAGACCTATCCGACGA/ACAGTTGGCGTTCAGGGAGCAGATT	163	111.201	0.999
18S	18S ribosomal RNA	∖	CCGACACGGGGAGGTAGTGACAATAA/CGCTATTGGAGCTGGAATTACCGC	149	97.636	0.998
28S	28S ribosomal RNA	∖	GAATGCAGCTCAAAATGGGGTGG/GCGACTGACTTCAAGCGTTTCCCT	160	111.048	0.999