Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Volvariella volvacea Gene Expression under Different Conditions

Table 2

Expression stability of 12 V. volvacea reference genes as calculated by NormFinder.

Rank	NaCl	CuSO4	H2O2	Heat	Cold	pH 4	pH 9	Developmental Stage	Total
1	UBQ	UBQ	SPRYp	UBQ	MSF1	MSF1	18S	L-asp	SPRYp
	0.244	0.105	0.164	0.033	0.070	0.132	0.029	0.287	0.354
2	SPRYp	TUBα	TUBβ1	MSF1	SPRYp	TUBα	L-asp	TUBβ1	TUBα
	0.253	0.18	0.269	0.083	0.072	0.226	0.139	0.288	0.373
3	CYP	SPRYp	ACTB	CYP	TUBα	TUBβ1	CYP	MAPK	MSF1
	0.425	0.205	0.367	0.304	0.104	0.253	0.250	0.315	0.485
4	L-asp	L-asp	TUBα	TUBβ1	ACTB	ACTB	UBQ	TUBα	L-asp
	0.524	0.288	0.403	0.399	0.365	0.365	0.267	0.316	0.553
5	TUBα	TUBβ1	UBQ	ACTB	L-asp	MAPK	MSF1	GAPDH	CYP
	0.534	0.301	0.467	0.410	0.400	0.379	0.297	0.323	0.557
6	18S	ACTB	MSF1	TUBα	UBQ	SPRYp	MAPK	18S	GAPDH
	0.674	0.396	0.539	0.439	0.450	0.390	0.319	0.337	0.752
7	ACTB	MSF1	L-asp	SPRYp	CYP	GAPDH	TUBα	SPRYp	MAPK
	0.777	0.623	0.615	0.497	0.459	0.436	0.320	0.417	0.826
8	GAPDH	18S	MAPK	GAPDH	28S	28S	TUBβ1	MSF1	UBQ
	0.803	0.669	0.645	0.637	0.550	0.473	0.365	0.423	0.831
9	MSF1	GAPDH	GAPDH	L-asp	MAPK	CYP	SPRYp	CYP	TUBβ1
	0.872	0.805	0.853	0.671	0.625	0.480	0.498	0.455	0.838
10	MAPK	CYP	18S	18S	GAPDH	18S	GAPDH	28S	28S
	0.945	0.816	0.872	0.883	0.902	0.490	0.601	0.577	0.843
11	TUBβ1	MAPK	CYP	28S	TUBβ1	UBQ	28S	UBQ	18S
	1.221	0.843	0.899	1.142	1.315	0.544	0.769	1.237	1.041
12	28S	28S	28S	MAPK	18S	L-asp	ACTB	ACTB	ACTB
	1.246	0.902	0.979	1.468	1.722	0.661	1.025	1.702	1.207