Research Article
Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Volvariella volvacea Gene Expression under Different Conditions
Table 3
Expression stability ranking of the 12 candidate reference genes.
| | Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| | RANKING ORDER UNDER NaCl STRESS (BETTER-GOOD-AVERAGE) | | geNorm | CYP | | SPRYp | TUBα | L-asp | GAPDH | ACTB | MSF1 | 18S | MAPK | 28S | TUBβ1 | | UBQ | | NormFinder | UBQ | SPRYp | CYP | L-asp | TUBα | 18S | ACTB | GAPDH | MSF1 | MAPK | TUBβ1 | 28S | | BestKeeper | SPRYp | UBQ | MAPK | L-asp | MSF1 | CYP | TUBα | ACTB | TUBβ1 | GADPH | 18S | 28S | | Comprehensive Ranking | UBQ | SPRYp | CYP | L-asp | TUBα | MAPK | MSF1 | ACTB | GAPDH | 18S | TUBβ1 | 28S | | RANKING ORDER UNDER H2O2 STRESS (BETTER-GOOD-AVERAGE) | | geNorm | TUBα | | SPRYp | ACTB | UBQ | MSF1 | MAPK | L-asp | GAPDH | CYP | 18S | 28S | | TUBβ1 | | NormFinder | SPRYp | TUBβ1 | ACTB | TUBα | UBQ | MSF1 | L-asp | MAPK | GAPDH | 18S | CYP | 28S | | BestKeeper | MAPK | MSF1 | UBQ | SPRYp | TUBβ1 | L-asp | ACTB | TUBα | CYP | GADPH | 28S | 18S | | Comprehensive Ranking | TUBβ1 | SPRYp | TUBα | MAPK | MSF1 | UBQ | ACTB | L-asp | GAPDH | CYP | 18S | 28S | | RANKING ORDER UNDER CuSO4 STRESS (BETTER-GOOD-AVERAGE) | | geNorm | TUBα | | TUBβ1 | ACTB | L-asp | SPRYp | CYP | GAPDH | MSF1 | 18S | MAPK | 28S | | UBQ | | NormFinder | UBQ | TUBα | SPRYp | L-asp | TUBβ1 | ACTB | MSF1 | 18S | GAPDH | CYP | MAPK | 28S | | BestKeeper | SPRYp | L-asp | UBQ | TUBβ1 | TUBα | MSF1 | MAPK | ACTB | CYP | GADPH | 18S | 28S | | Comprehensive Ranking | UBQ | TUBα | SPRYp | L-asp | TUBβ1 | ACTB | MSF1 | CYP | GAPDH | MAPK | 18S | 28S | | RANKING ORDER UNDER COLD STRESS (BETTER-GOOD-AVERAGE) | | geNorm | MSF1 | | TUBα | ACTB | L-asp | UBQ | CYP | 28S | GAPDH | MAPK | TUBβ1 | 18S | | SPRYp | | NormFinder | MSF1 | SPRYp | TUBα | ACTB | L-asp | UBQ | CYP | 28S | MAPK | GAPDH | TUBβ1 | 18S | | BestKeeper | GADPH | UBQ | L-asp | CYP | MSF1 | SPRYp | TUBα | MAPK | ACTB | 28S | TUBβ1 | 18S | | Comprehensive Ranking | MSF1 | SPRYp | TUBα | UBQ | L-asp | GAPDH | ACTB | CY11P | 28S | MAPK | TUBβ1 | 18S | | RANKING ORDER UNDER HOT STRESS (BETTER-GOOD-AVERAGE) | | geNorm | UBQ | | CYP | ACTB | TUBβ1 | TUBα | L-asp | GAPDH | SPRYp | 18S | 28S | MAPK | | MSF1 | | NormFinder | UBQ | MSF1 | CYP | TUBβ1 | ACTB | TUBα | SPRYp | GAPDH | L-asp | 18S | 28S | MAPK | | BestKeeper | MSF1 | TUBα | L-asp | GADPH | SPRYp | TUBβ1 | CYP | 18S | ACTB | UBQ | MAPK | 28S | | Comprehensive Ranking | MSF1 | UBQ | CYP | TUBα | TUBβ1 | ACTB | L-asp | GAPDH | SPRYp | 18S | 28S | MAPK | | RANKING ORDER UNDER ACID STRESS (BETTER-GOOD-AVERAGE) | | geNorm | SPRYp | | MSF1 | TUBβ1 | TUBα | 28S | 18S | GAPDH | ACTB | CYP | UBQ | L-asp | | MAPK | | NormFinder | MSF1 | TUBα | TUBβ1 | ACTB | MAPK | SPRYp | GAPDH | 28S | CYP | 18S | UBQ | L-asp | | BestKeeper | TUBβ1 | MAPK | SPRYp | MSF1 | TUBα | L-asp | CYP | UBQ | ACTB | GADPH | 28S | 18S | | Comprehensive Ranking | MAPK | MSF1 | TUBβ1 | SPRYp | TUBα | ACTB7 | 28S | GAPDH | CYP | 18S | L-asp | UBQ | | RANKING ORDER UNDER ALKALI STRESS (BETTER-GOOD-AVERAGE) | | geNorm | MSF1 | | 18S | L-asp | CYP | UBQ | TUBα | TUBβ1 | SPRYp | GAPDH | 28S | ACTB | | MAPK | | NormFinder | 18S | L-asp | CYP | UBQ | MSF1 | MAPK | TUBα | TUBβ1 | SPRYp | GAPDH | 28S | ACTB | | BestKeeper | L-asp | MSF1 | MAPK | SPRYp | GADPH | CYP | TUBα | UBQ | TUBβ1 | 18S | ACTB | 28S | | Comprehensive Ranking | L-asp | MSF1 | MAPK | 18S | CYP | UBQ | SPRYp | TUBα | GAPDH | TUBβ1 | 28S | ACTB | | RANKING ORDER UNDER DIFFERENT STAGES (BETTER-GOOD-AVERAGE) | | geNorm | TUBβ1 | | TUBα | L-asp | 18S | GAPDH | SPRYp | MSF1 | CYP | 28S | UBQ | ACTB | | MAPK | | NormFinder | L-asp | TUBβ1 | MAPK | TUBα | GAPDH | 18S | SPRYp | MSF1 | CYP | 28S | UBQ | ACTB | | BestKeeper | SPRYp | GADPH | L-asp | CYP | UBQ | MAPK | MSF1 | TUBα | TUBβ1 | 18S | 28S | ACTB | | Comprehensive Ranking | L-asp | TUBβ1 | MAPK | SPRYp | GAPDH | TUBα | 18S | CYP | MSF1 | UBQ4 | 28S | ACTB | | RANKING ORDER UNDER ALL SAMPLES (BETTER-GOOD-AVERAGE) | | geNorm | TUBα | | L-asp | CYP | GAPDH | MSF1 | UBQ | 28S | MAPK | TUBβ1 | 18S | ACTB | | SPRYp | | NormFinder | SPRYp | TUBα | MSF1 | L-asp | CYP | GAPDH | MAPK | UBQ | TUBβ1 | 28S | 18S | ACTB | | BestKeeper | CYP | L-asp | MSF1 | MAPK | GADPH | ACTB | SPRYp | TUBβ1 | TUBα | UBQ | 18S | 28S | | Comprehensive Ranking | SPRYp | TUBα | CYP | L-asp | MSF1 | GAPDH | MAPK | UBQ | TUBβ1 | ACTB | 28S | 18S |
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