Natural Compound Oridonin Inhibits Endotoxin-Induced Inflammatory Response of Activated Hepatic Stellate Cells

Figure 3

Oridonin affected LPS-stimulated IKKα/β and IκBα phosphorylation and IκBα degradation. LX-2 cells were treated with 7.5 μM of oridonin for 1 h followed by LPS (1 μg/mL) for 30 min. Total proteins were examined by western blots using antibody pIκBα and IκBα (a), or pIKKα/β, IKKα and IKKβ (b). GAPDh was used as internal control. Densitometric analyses of bands were quantified and data expressed as fold of control normalized to GAPDH. All Western blot pictures are the representative of at least 3 independent experiments.

(a)

(b)