Natural Compound Oridonin Inhibits Endotoxin-Induced Inflammatory Response of Activated Hepatic Stellate Cells

Figure 5

The inhibitory effects of PES-CI on LPS-induced NF-κB signaling was enhanced by oridonin. LX-2 cells were pretreated with 2.5 μM of oridonin, PES-CI or combination of two agents for 1 h, then stimulated with LPS (1 μg/mL) for 30 min. Nuclear protein was isolated for Western blot assay using NF-κB p65 antibody. Lamin A was used as internal control to normalize the nuclear p65 protein (a). The subcellular translocation of NF-κB p65 was imaged by immunofluorescence assay (b). DNA binding activity of NF-κB p65 was analyzed by TransAM p65 ELISA kit with 10 μg of nuclear proteins (c). Total proteins were examined by Western blots using antibody pIκBα, IκBα, or pIKKα/β (d). Densitometric analyses of bands were quantified and data expressed as fold of control normalized to GAPDH. Data are representative of 3 independent experiments.

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