Silibinin Induced Human Glioblastoma Cell Apoptosis Concomitant with Autophagy through Simultaneous Inhibition of mTOR and YAP

Figure 2

Silibinin induced apoptosis in glioblastoma cells. (a) Glioblastoma cells were treated with different concentrations of silibinin for 48 h; then the apoptotic index was calculated after flow cytometric analysis. (b) After treatment with different concentrations of silibinin for 48 h, total lysates of A172 and SR cells were analyzed for caspase 3, cleaved caspase 3, and PARP cleavage by western blotting. β-Actin was used as a loading control. Data are presented as mean ± SD ( in each group).

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