Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized Talaromyces thermophilus Lipase

Figure 1

The effect of different resins on immobilization yield and activity of immobilized enzyme. Epoxy resin was directly added to the crude fermentation lipase under gentle stirring at room temperature. The reaction mixture consisted of 10 mL Tris-HCl buffer (100 mM, pH 8.0), 5 mM zinc acetate, 100 mM CNDE, and 0.2 g immobilized lipase. The reaction was performed at 30°C and stirred at 500 rpm. Symbols: filled square, relative activity of immobilized lipase; bar, immobilization yield of epoxy resin.