Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized<i> Talaromyces thermophilus</i> Lipase

<div>Effect of protein/epoxy resin ratio on immobilization yield and activity of the immobilized TTL mutant. The epoxy resin D5730 was directly added to the crude lipase with different protein/epoxy resin ratio under gentle stirring at room temperature. The reaction was performed at 30°C and stirred at 500 rpm using immobilized enzyme with 100 mM CNDE and 5 mM zinc acetate in Tris-HCl buffer. Symbols: filled square, relative activity of immobilized enzyme; square, protein loading.</div>

BioMed Research International

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Figure 2: Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized<i> Talaromyces thermophilus</i> Lipase 

BioMed Research International

Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized Talaromyces thermophilus Lipase

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