Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized<i> Talaromyces thermophilus</i> Lipase

<div>Progress curves of kinetic resolution of CNDE catalyzed by immobilized TTL mutant in Tris-HCl buffer. Symbols: filled square, 1 M CNDE; filled circle, 2 M CNDE; filled triangle, 3 M CNDE; square, <svg height="11.2615pt" id="M14" style="vertical-align:-5.300591pt" version="1.1" viewbox="-0.0498162 -5.96091 16.2145 11.2615" width="16.2145pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M391 364C391 409 353 448 295 448C249 448 198 426 152 393C65 331 23 225 23 139C23 14 96 -12 146 -12C198 -12 280 9 367 101L351 124C300 78 242 48 194 48C129 48 109 107 109 162V191C208 213 391 266 391 364ZM313 350C313 305 268 261 113 223C132 334 187 381 217 398C227 404 244 405 261 405C290 405 313 385 313 350Z"></path></g><g transform="matrix(.013,0,0,-0.013,5.382,0)"><path d="M391 364C391 409 353 448 295 448C249 448 198 426 152 393C65 331 23 225 23 139C23 14 96 -12 146 -12C198 -12 280 9 367 101L351 124C300 78 242 48 194 48C129 48 109 107 109 162V191C208 213 391 266 391 364ZM313 350C313 305 268 261 113 223C132 334 187 381 217 398C227 404 244 405 261 405C290 405 313 385 313 350Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,10.699,3.132)"><path d="M173 382V461C125 442 68 425 25 418V390C88 382 92 382 92 317V-127C92 -191 84 -199 18 -204V-235H259V-204C180 -196 173 -192 173 -120V7C188 -1 212 -11 243 -12C375 12 495 109 495 261C495 361 427 451 316 451C303 451 283 446 266 435L173 382ZM173 345C200 365 243 388 274 388C346 388 409 329 409 222C409 111 353 40 266 40C231 40 196 56 173 79V345Z"></path></g></svg> of<i> S</i>-CCMA.</div>

BioMed Research International

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Figure 5

Figure 5: Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized<i> Talaromyces thermophilus</i> Lipase 

BioMed Research International

Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized Talaromyces thermophilus Lipase

Figure 5