The Use of Phage Display and Yeast Based Expression System for the Development of a Von Willebrand Factor Propeptide Assay: Development of a Von Willebrand Factor Propeptide Assay

Box 1

Protein sequence of antibody fragments JA9 and JG7. The differences between the two antibody fragments are underlined.

JG7

PIT2 vector: DSGNSYDHDYAKLACKFYFKETVIMKYLLPTAAAGLLLLAAQPAMAEV

Lightchain:QLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSSIVETGPETSYADSVKGRFTIS

     RDNSKNTLYLQMNSLRAEDTAVYYCAKSPQL

Linker sequence:FDYWGQGTLVTVSSGGGGSGGGGSGGGGSTDIQMTQ

Heavychain:SPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYRASSLQSGVPSRFSGSGSGTDFTLTIS

    SLQPEDFATYYCQQGHPTPGTFGQGTKVEIKR

PIT2 vector:AAAHHHHHHGAAEQKLISEEDLNGAAEITS

JA9

PIT2 vector:DSGNSYDHDYAKLACKFYFKETVIMKYLLPTAAAGLLLLAAQPAMAEV

Lightchain:QLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSSIYEEGVLTMYADSVKGRFTIS

     RDNSKNTLYLQMNSLRAEDTAVYYCAKRSVIR

Linker: FDYWGQGTLVTVSSGGGGSGGGGSGGGGSTDIQMTQ

Heavychain:SPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASCKVGSPSRFSGSGSGTDFTLTISS

     LQPEDFATYYCQQENCEYTFGQGTKVEIKR

PIT2 sequence: AAAHHHHHHGAAEQKLISEEDLNGAXEITS