Anthocyanins Function as Anti-Inflammatory Agents in a Drosophila Model for Adipose Tissue Macrophage Infiltration

Figure 1

Obese larvae have increased hemocytes infiltrating the fat cells. (a) Ecdysone regulation of larval molting and metamorphosis. Reducing the size of the ring gland reduces ecdysone level in P0206-Gal4; UAS-Ni animals. (b) Quantitative RT-PCR in whole larvae of the indicated genotype showing the relative expression of E74b mRNA. Actin5c was used as control. (c) Relative size of cells from the FBs from animals of the indicated genotypes, at 5 and 12 days AEL. (d) Nile Red staining for lipids, Phalloidin for membranes, and Hoechst for nuclei, of FBs. (e) % of hemocytes infiltrating the FBs of animals at 5 or 12 days AEL, of the indicated genotype. Data are expressed as percentage of hemocytes in the cells of FBs. (f) Draw and confocal photographs of cell from the FB, showing hemocytes stained with anti-SPARC antibodies (red), while nuclei are visualized using Hoechst (blue). Error bars represent SEM (standard error of the mean) of three independent experiments. and .

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