Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips

Figure 2

Screening of primer combinations. Sixteen groups of primers (indicated in the table) were used to conduct the RPA-LF reaction with recombinant plasmid 23SrRNA-pUC19 as positive template (P) and plasmid pUC19 as negative template (N). Group 17 using primers, probe, and template from the TwistAmp RPA nfo kit was conducted as a control. SP, sample pad of the strip.