Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips

Figure 3

Optimization of the RPA-LF detection system. (a) Various concentrations of reverse primer and probe were combined to make 6 groups as indicated in Table 3 and evaluated using RPA-LF; (b) various volumes (1 μL, 2 μL, or 5 μL) of the amplified products were used to develop the MGH strips; (c) various of amplification times (10, 15, or 20 min) were used in RPA-LF and developed with MGH strips. Each group used both recombinant plasmid 23SrRNA-pUC19 as positive template (P) and plasmid pUC19 as negative template (N).

(a)

(b)

(c)