Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips

Figure 4

Sensitivity analysis of RPA-LF detection method. Sensitivity of RPA-LF detection method was evaluated with various copies of 23SrRNA-pUC19 (a) or various of dilutions of genomic DNA of C. burnetii (b). Copies of genomic DNA of dilutions 1 to 5 (as shown on (b)) were evaluated as undetected, 7 copies/μL, 51 copies/μL, 1002 copies/μL, and 14526 copies/μL, respectively, by RT-qPCR (c). N, negative control using template of pUC19 plasmid (a) or human blood DNA (b).

(a)

(b)

(c)